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질량 분석기 분석을위한 펩티드로 조직과 소화에서 세포 외 기질 단백질의 농축
JoVE 신문
생물학
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JoVE 신문 생물학
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
DOI:

07:28 min

July 23, 2015

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Chapters

  • 00:05Title
  • 01:13Decellularization of the Tissue
  • 04:27Digestion of the ECM Proteins into Peptides
  • 06:11Results: Analysis of the Decellularization Process by Western Blot
  • 07:03Conclusion

Summary

자동 번역

This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

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