JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:37Plate Seeding with a Manual Multiple-channel Pipetter
- 02:33Plate Seeding with a Robotic Pipetter
- 05:23Plate Drugging Using a Robotic Pipettor and Seeded Cell Imaging
- 07:36Results: Representative Robotic Seeding and Drug Treatment of MM Cells
- 09:01Conclusion
We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.
