Journal
/
/
Использование CaCO 2-Cells по изучению транспорта липидов в кишечнике
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Using Caco-2 Cells to Study Lipid Transport by the Intestine
DOI:

07:00 min

August 20, 2015

,

Chapters

  • 00:05Title
  • 01:07Maintenance of Caco-2 Cells
  • 02:24Stimulating Lipoprotein Secretion and Lipoprotein Isolation
  • 04:35Transmission Electron Microscopy Analysis
  • 05:42Results: Analysis of Lipoproteins Secreted by Caco-2 Cells
  • 06:35Conclusion

Summary

자동 번역

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

Related Videos

Read Article