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Chapters
- 00:05Title
- 00:32Enzymatic Processing of Plasmids
- 03:06Fluorescence Measurements for a Chemical Reaction A + B -> B + C
- 06:13Results: Fluorescence Kinetics Measurements (Plasma-derived vs. Synthesized Gates)
- 07:24Conclusion
This protocol describes a method for deriving DNA strand displacement gates from plasmids and testing them using fluorescence kinetics measurements. Gates can be modularly composed into multi-component systems to approximate the behavior of formal chemical reaction networks (CRN), demonstrating a new use for CRNs as a molecular programming language.
