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Chapters
- 00:05Title
- 01:27Production of Dorsal Substrates
- 02:52Sandwich Culture
- 03:55Wound Healing Assay
- 04:55Results: Sandwich-like Culture Strongly Influences Cell Morphology, Cell Differentiation and ECM Reorganization
- 06:18Conclusion
The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
