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Chapters
- 00:05Title
- 01:45Preparation of Silicon Nitride Membrane Microchips
- 02:56Preparation of Cells on Microchips
- 03:52EGFR Labeling, Fixation, and Fluorescence Microscopy
- 06:12Wet Environmental Scanning Electron Microscopy-Scanning Transmission Electron Microscopy of Whole Cells (ESEM-STEM)
- 07:20Results: EGFR Stoichiometry in Labeled COS7 Cells
- 08:45Conclusion
This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.
