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Chapters
- 00:05Title
- 01:16Bio-chip Overview
- 02:53Preparation of Large Unilamellar Vesicles (LUVs)
- 04:45Protein Reconstitution
- 06:14Bio-chip Holder Preparation for the Translocation Assay
- 08:29The Translocation Assay
- 09:47Results: Quality Control Tests and Experimental Manipulations
- 11:28Conclusion
The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector screenings.
