JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:57Generation of Neonatal Mouse Cardiac Fibroblasts via Explant Culture
- 03:20Generation of Neonatal Mouse Cardiac Fibroblasts via Enzymatic Digestion
- 05:02Isolation of Thy1.2+ Fibroblasts by Magnetic-activated Cell Sorting (MACS)
- 07:17Retroviral Mediated Reprogramming of Isolated Cardiac Fibroblasts
- 08:52Results: Retroviral Reprogramming of Cardiac Fibroblasts to Induced Cardiac Myocytes
- 09:38Conclusion
We describe here a protocol for the generation of iCMs using retrovirus-mediated delivery of Gata4, Tbx5 and Mef2c in a polycistronic construct. This protocol yields a relatively homogeneous population of reprogrammed cells with improved efficiency and quality and is valuable for future studies of iCM reprogramming.
