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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
 

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Article DOI: 10.3791/53508-v • 11:17 min • January 7th, 2016
January 7th, 2016 •
*1, *1, 2, 3
1Department of Nanoscience, University of North Carolina at Greensboro, 2Center for Biotechnology, Genomics, and Health Research, University of North Carolina at Greensboro, 3HORIBA Scientific
* These authors contributed equally

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The proposed work assesses the diagnostic potential of direct and amplified surface plasmon resonance imaging (SPRi) assays, particularly for the detection of recombinant human growth hormone in spiked human serum, by comparing SPRi results directly with commercially available enzyme-linked immunosorbent assay (ELISA) kit.

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Keywords: SPRi Nano-SPRi ELISA HGH Serum Biomarker Detection Label-free Real-time Monitoring Biochip Surface Chemistry 11-mercaptoundecanoic Acid 1-Ethyl-3-carbodiimide N-Hydroxysuccinimide
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