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人类成胶质细胞瘤器官型切片培养模型，用于研究肿瘤细胞迁移和抗创伤药物的患者特异性作用

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A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

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人类成胶质细胞瘤器官型切片培养模型，用于研究肿瘤细胞迁移和抗创伤药物的患者特异性作用

DOI:

10.3791/53557-v

•

08:35 min

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July 20, 2017

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Jonathon J. Parker, Marcela Lizarraga, Allen Waziri, Kara M. Foshay

¹Department of Neurosurgery,Stanford University Hospital, Stanford University School of Medicine, ²Inova Neuroscience Institute

Chapters

00:05Title
00:43Slice Culture Acquisition and Maintenance
03:41GFP-expressing Retrovirus Glioblastoma (GBM) Cell Labeling and Time-lapse Single Photon Laser Scanning Confocal Imaging
05:02Imaging Post-processing and Tumor Cell Tracking
06:53Results: Representative Human GBM Imaging and Analyses
08:15Conclusion

Summary

자동 번역

目前的胶质母细胞瘤（GBM）的离体模型并没有针对人肿瘤浸润的生理学相关研究进行优化。在这里，我们提出了新鲜人类GBM组织的器官切片培养物的产生和维持方案。提供延时显微术和定量细胞迁移分析技术的描述。

Tags

Keywords: Glioblastoma Organotypic Slice Culture Tumor Cell Migration Anti-invasive Drugs Neurooncology Primary Cells Cellular Heterogeneity Agarose Embedding Vibratome Sectioning

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