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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
 

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Article DOI: 10.3791/53611-v • 12:14 min • March 9th, 2016
March 9th, 2016 •
1, 1, 1
1Department of Microbiology, Southern California Costal Water Research Project Authority

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This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

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Duplex Digital PCR Assay Quantification Enterococcus Spp. Human Fecal-associated HF183 Marker Waters Fecal Contamination Recreational Water Quality Monitoring Assay Advantages QPCR Standard Curves Bias Variability Procedure Demonstration Stock Concentrations Primers Molecular Grade Water Probes TEPH 8 Buffer Master Mix Preparation Pipetting Technique Homogenous Solution Assay Mixture Droplet Gen
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