Journal
/
화학
/
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

DOI:
10.3791/53644-v

•

09:05 min

•

January 06, 2016

•

,
1Department of Chemistry,University at Albany, State University of New York, 2Multiplex Biotechnology Laboratory,Cancer Research Center

Chapters

  • 00:05Title
  • 00:44One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
  • 02:21One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
  • 03:39One-dimensional DNA Barcode Slides: Patterning of ssDNA
  • 05:00Two-dimensional 3×3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
  • 06:17Two-dimensional 3×3 DNA Arrays: Validation and Immunoassay
  • 07:32Results: Multiplex-protein Detection from Single-cell Preparations
  • 08:25Conclusion

Summary

자동 번역

자동 번역

This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.

Tags

Flow-patternBarcodeAntibody MicroarraySingle-cell AnalysisPDMS MoldSU-8 MasterPhoto-maskPoly-L-lysinePolyethylene TubingStainless Steel Pins

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/53644?article_type=v

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
check_url/kr/53644?article_type=v

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
check_url/kr/53644?article_type=v

Fabrication of Large-area Free-standing Ultrathin Polymer Films
check_url/kr/53644?article_type=v

Microbubble Fabrication of Concave-porosity PDMS Beads
check_url/kr/53644?article_type=v

Influence of Hybrid Perovskite Fabrication Methods on Film Formation, Electronic Structure, and Solar Cell Performance
check_url/kr/53644?article_type=v

Fabrication and Testing of Photonic Thermometers
check_url/kr/53644?article_type=v

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
check_url/kr/53644?article_type=v

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction
check_url/kr/53644?article_type=v

Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941
check_url/kr/53644?article_type=v

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

Read Article