JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:01Infection of A549 Cells
- 03:36Preparation of Whole Cell Extracts
- 05:07Resolution of Whole Cell Extracts by High Resolution SDS-PAGE
- 06:20Analysis of IRF3 Dimerization by Native-PAGE
- 07:37Immunoblot Analysis of IRF3 Species
- 09:23Results: Detection Distinct Phosphorylated IRF3 Species
- 10:56Conclusion
Here we describe a procedure allowing a detailed analysis of the phosphorylation-dependent activation of the IRF3 transcription factor. This is achieved through the combination of a high resolution SDS-PAGE and a native-PAGE coupled to immunoblots using multiple phosphospecific antibodies.
