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JoVE Journal
Developmental Biology

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Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
 

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Article DOI: 10.3791/53966-v 13:01 min April 10th, 2016
April 10th, 2016

챕터

요약

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

Tags

Keywords: Light Sheet Fluorescence Microscopy Zebrafish Eye Development Multiview Reconstruction Low Phototoxicity Fast Acquisition 3D Volume Developmental Biology Neuroscience Agarose E3 Medium MS 222 Fluorescent Beads
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