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心筋細胞のDNA合成と倍数性の同時評価:心筋細胞再生の定量化を支援する方法および売上高
JoVE 신문
발생학
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JoVE 신문 발생학
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
DOI:

08:03 min

May 23, 2016

Chapters

  • 00:05Title
  • 00:48Heart Harvest
  • 02:13Nuclei Dissociation and Cardiomyocyte Nuclei Labeling
  • 04:51EdU Incorporation Detection
  • 06:05Results: EdU Labeled Cardiomyocyte Quantification in WT and Mdx Hearts
  • 07:03Conclusion

Summary

자동 번역

Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.

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