Journal
/
화학
/
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

DOI:
10.3791/54157-v

•

09:14 min

•

July 20, 2016

•

,
1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,The University of New South Wales

Chapters

  • 00:05Title
  • 00:43Purification of Cytochrome c
  • 02:11Synthesis of Cytochrome c Bioconjugates
  • 03:47Purification of Cytochrome c Bioconjugates
  • 06:02Investigation of Bioconjugates by Gel Electrophoresis
  • 07:08Results: Synthesis and Characterization of Cytochrome c Bioconjugates
  • 08:02Conclusion

Summary

자동 번역

자동 번역

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

Tags

Protein BioconjugatesCysteine-maleimide ChemistrySite-specific ModificationAntibody Drug ConjugatesNano-medicineFluorescent MicroscopySystems ChemistryCytochrome CFPLCRuthenium Maleimide ComplexTCEP

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54157?article_type=v

Synthesis of Antiviral Tetrahydrocarbazole Derivatives by Photochemical and Acid-catalyzed C-H Functionalization via Intermediate Peroxides (CHIPS)
check_url/kr/54157?article_type=v

Synthesis and Characterization of Functionalized Metal-organic Frameworks
check_url/kr/54157?article_type=v

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
check_url/kr/54157?article_type=v

Synthesis and Reaction Chemistry of Nanosize Monosodium Titanate
check_url/kr/54157?article_type=v

The Synthesis of [Sn10(Si(SiMe3)3)4]2– Using a Metastable Sn(I) Halide Solution Synthesized via a Co-condensation Technique
check_url/kr/54157?article_type=v

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
check_url/kr/54157?article_type=v

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism
check_url/kr/54157?article_type=v

A Rapid Synthesis Method for Au, Pd, and Pt Aerogels Via Direct Solution-Based Reduction
check_url/kr/54157?article_type=v

Synthesis of Esters Via a Greener Steglich Esterification in Acetonitrile
check_url/kr/54157?article_type=v

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Read Article