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Chapters
- 00:05Title
- 00:46Stage 1 and 2: HEK Cell Culture and Production and Concentration of Lentiviruses in HEK Cells
- 04:46Stage 3 and 4: Culture and Transduce Primary HBE Cells
- 07:20Results: Lentiviral Transduction of Primary Human Bronchial Epithelial Cells
- 08:06Conclusion
Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.
