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Developmental Biology

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JoVE Journal Developmental Biology
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
 

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Article DOI: 10.3791/54228-v • 12:02 min • July 30th, 2016
July 30th, 2016 •
*1, *1, *1, 1, 1, 1, 1, 1, 1, 1
1INRS-Institut Armand-Frappier
* These authors contributed equally

챕터

요약
Automatic Translation

This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

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Keywords: Primary Trophoblast Cell Culture Hypoxia/reoxygenation Melatonin Pregnancy Complications Placenta Oxidative Stress Villous Cytotrophoblast Isolation Density Centrifugation Trophoblast Culture First Trimester Placenta Placental Tissue Digestion
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