JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Molecular Imaging and Neurovascular Research Laboratory,Dongguk University College of Medicine, 2Biomedical Research Center,Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering,Seoul National University, 4Departments of Radiology and Cancer Systems Imaging,University of Texas M.D. Anderson Cancer Center
Chapters
- 00:05Title
- 01:20Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
- 02:55Thromboembolic Stroke Model Preparation
- 05:42In Vivo MicroCT Cerebral Thrombus Imaging
- 06:36Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
- 09:48Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
- 11:28Conclusion
Detta protokoll beskriver tillämpningen av kombinerade nära infraröda fluorescerande (NIRF) avbildning och mikro datortomografi (microCT) för visualisering cerebral tromboemboli. Denna teknik medger kvantifiering av trombbördan och evolution. Den NIRF avbildningsteknik visualiserar fluorescensmärkt tromb i utskuren hjärnan, medan microCT tekniken visualiserar trombos inuti levande djur med hjälp av guldnanopartiklar.
