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Chapters
- 00:05Title
- 00:45Synthesis and Operating Principles of the ‘Chemical Transducer’
- 01:51Setting Up the Plate Reader for Kinetic Measurement
- 03:26Measuring GST Activity in the Presence of the ‘Chemical Transducer’ and PDGF
- 04:59Evaluating GST Activation/Inhibition Cycles Mediated by the ‘Chemical Transducer’
- 06:37Evaluating the Real-time Effect of PDGF Addition
- 07:42Measuring JS-K Prodrug Activation by GST in the Presence of the ‘Chemical Transducer’ and PDGF
- 10:16Results: ‘Chemical Transducer’ Induces Artificial Communication Between PDFG and GST
- 11:53Conclusion
We present guidelines for developing synthetic 'chemical transducers' that can induce communication between naturally unrelated proteins. In addition, detailed protocols are presented for synthesizing and testing a specific 'transducer' that enables a growth factor to activate a detoxifying enzyme and consequently, to regulate the cleavage of an anticancer prodrug.
