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Chapters
- 00:05Title
- 01:11Isolation and Culture of Primary Tubal Epithelium Cells
- 02:26Preparation of Uterine Tube and Ovarian Fat Pad Transplantation
- 05:35Graft Collection and Preservation
- 06:36Results: Transplantation into the Mouse Ovarian Fat Pad
- 08:15Conclusion
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
