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Developmental Biology

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JoVE Journal Developmental Biology
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
 

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Article DOI: 10.3791/54650-v • 09:57 min • December 21st, 2016
December 21st, 2016 •
1, 1, 2
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

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요약
Automatic Translation

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.

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Keywords: Induced Pluripotent Stem Cells IPSCs Peripheral Blood Mononuclear Cells PBMCs Reprogramming Centrifugation Sendai Virus Blood Cell Media Density Gradient Media Cell Isolation Cell Culture Cell Counting Transduction
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