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在用水筛查内分泌活动市售的<em>体外</em>式激活生物测定

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Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

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式激活生物测定

DOI:

10.3791/54725-v

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08:00 min

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December 04, 2016

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Alvine C. Mehinto, B. Sumith Jayasinghe, Darcy R. Vandervort, Nancy D. Denslow, Keith A. Maruya

¹Southern California Coastal Water Research Project Authority, ²Department of Physiological Sciences,University of Florida

Chapters

00:05Title
01:00Prepare Dilutions of the Assay-specific Reference Chemical and Water Extract
02:42Prepare the Cell Suspension to Conduct the Fret Bioassay
04:05Plate Cells in a 96-well Black Wall Clear-bottom Plate and Add Diluted Water Extract
04:54Measure Cytotoxicity and Endocrine Activity Response
05:51Results
07:22Conclusion

Summary

자동 번역

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

在用水筛查内分泌活动市售的<em>体外</em>式激活生物测定

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Chapters

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자동 번역

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