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JoVE Journal Environment
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
 

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Article DOI: 10.3791/54741-v • 08:04 min • November 25th, 2016
November 25th, 2016 •
1, 2, 3, 4, 4, 2, 1, 5
1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo

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요약
Automatic Translation

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

Tags

Filter Cartridge Water Samples Environmental DNA Filtration Metabarcoding Aquatic Organisms Biodiversity Monitoring Community Ecology Larger Water Volumes Sterile Manner Aspirator Pump Manifold Vacuum Tubing Luer Fitting Vacuum Connector Sampled Water Plastic Bag Screw Cap Male Luer-lock Connector Mesh Panel
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