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在线体积排阻和离子交换色谱法SAXS光束线
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
DOI:

11:09 min

January 05, 2017

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Chapters

  • 00:05Title
  • 00:51Size-exchange Chromatography (SEC)-Small Angle X-ray Scattering (SAXS) Data Collection
  • 03:46Ion-exchange Chromatography (IEC)-SAXS Data Collection
  • 07:37SAXS Data Reduction and Analysis
  • 08:59Results: SEC- and IEC-SAXS Analysis of D5323-785
  • 09:55Conclusion

Summary

자동 번역

通过小角X射线散射(SAXS)的蛋白的溶液结构的确定需要单分散样品。在这里,我们提出了两种可能性,以确保样品制备和数据采集之间的最小延迟:在线尺寸排阻色谱(SEC)和在线离子交换层析(IEC)。

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