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Chapters
- 00:05Title
- 01:15Heart Harvesting and Tissue Preparation
- 04:07Masson-Goldner Trichrome Staining
- 06:06Infarct Size Analysis
- 08:19Results: Correlation between the Expansion Index (EI) and the Percentage of the Infarct Related to LV (A) or the Ejection Fraction
- 09:14Conclusion
The post-mortem assessment of myocardial infarction (MI) in rodents is based on quantification of the infarct on stained heart sections. We describe an accurate method to quantify the infarct size using systematic sampling of harvested rat hearts from base to apex and image analyses of trichrome-stained histological sections.
