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JoVE Journal
Biochemistry

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

SorLA en CLC
 
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SorLA en CLC: CLF-1-afhankelijke negatieve regulatie van CNTFRa zoals aangetoond door Western blotting, Remming van lysosomale enzymen en immunocytochemie

Article DOI: 10.3791/55019-v 10:16 min January 6th, 2017
January 6th, 2017

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요약

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Dit protocol beschrijft hoe Western blotting en immunocytochemie gecombineerd met remming van lysosomale enzymen kunnen worden gebruikt om de neerwaartse regulatie van ciliaire neurotrofe factor receptor-α (CNTFRa), die wordt overgebracht door de interactie tussen cytokine-achtige factor-1 (CLF-1 tonen ) en de endocytosereceptor sorLA.

Tags

Biochemie CLC: CLF-1 NNT-1 BSF-3 CRLF1 CNTFRa sorLA Vps10p STAT3
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