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Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
 

Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

Article DOI: 10.3791/55028-v 10:04 min January 8th, 2017
January 8th, 2017

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요약

Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.

Tags

Keywords: Tetraploid Cells Human Fibroblasts Carcinogenesis Research Aneuploid Cells Cell Culture Demecolcine Mitotic Cells Cell Counting Cell Reseeding
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