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1School of Natural Sciences,University of California, Merced, 2Centro de Investigaciones Cardiovasculares,Universidad de la Plata and Conicet, 3Facultad de Ingenieria,Universidad Nacional de Entre Rios, 4Department of Physiology,Midwestern University, 5School of Engineering,University of California, Merced
Chapters
- 00:05Title
- 00:45Preparation and Heart Harvest
- 03:50Dye Perfusion (Rhod-2AM for Cystosolic Ca2+ Measurements)
- 05:59Recording Epicardial and Endocardial Signals
- 08:41Results: AP and Ca2+ Transients in Endocardium and Epicardium
- 10:02Conclusion
In the heart, molecular events coordinate the electrical and contractile function of the organ. A set of local field fluorescence microscopy techniques presented here enables the recording of cellular variables in intact hearts. Identifying mechanisms defining the cardiac function is critical in understanding how the heart works under pathological situations.
