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Developmental Biology
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JoVE Journal Developmental Biology
Acute and Chronic Models of Hyperglycemia in Zebrafish
 

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

Article DOI: 10.3791/55203-v • 13:35 min • June 26th, 2017
June 26th, 2017 •
1, 1, 2, 1,2, 1,3, 1
1UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France, Université de La Réunion, INSERM, 2RIPA (Radiochimie et Imagerie du Petit Animal), Cyclotron de la Réunion Océan Indien CYROI, 3CHU de La Réunion

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요약
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This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.

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Keywords: Zebrafish Hyperglycemia Neurogenesis Biodistribution Radiolabeled Molecules Acute Hyperglycemia Chronic Hyperglycemia Diabetes Brain Remodeling Ischemic Stroke Animal Model Intraperitoneal Injection Blood Glucose Measurement
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