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JoVE Journal Biology
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
 

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D

Article DOI: 10.3791/55304-v • 11:27 min • March 16th, 2017
March 16th, 2017 •
*1, *1, 1, 1, 1, 1,2, 1,2, 1,2, 1
1Department of Medicine, University of Illinois at Chicago, 2Department of Research, Jesse Brown VA Medical Center
* These authors contributed equally

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요약
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We describe simple methods to study the regulation of intestinal serotonin transporter (SERT) function and expression using an in vitro cell culture model of Caco-2 cells grown in 3D and an ex vivo model of mouse intestines. These methods are applicable to the study of other epithelial transporters.

Tags

Epithelial Transport Protein Function Expression Native Intestine Caco-2 Cells 3D Culture Ussing Chamber Serotonin Transporter Regulation Cell-to-cell Interactions Cell-to-extracellular Matrix Interactions Monolayers Transport Function Measurements Therapeutics Discovery Electrolyte Transporters Sodium Transporters Chloride Transporters Seromuscular Layer Stripping Intestinal Mucosa Mounting
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