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Bioengineering

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JoVE Journal Bioengineering
Visualisere angiogenese af Multiphoton mikroskopi In Vivo i genetisk modificerede 3D-PLGA/nHAp stillads til Calvarial kritiske knogle defekt reparation
 
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Visualisere angiogenese af Multiphoton mikroskopi In Vivo i genetisk modificerede 3D-PLGA/nHAp stillads til Calvarial kritiske knogle defekt reparation

Article DOI: 10.3791/55381-v • 09:34 min • September 7th, 2017
September 7th, 2017 •
1, 2,3, 4, 1
1Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery, Maastricht UMC+, 3Department of Orthopaedic Surgery, University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences

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Vi præsenterer her, en protokol for at visualisere blodkar dannelse i vivo og i realtid i 3D stilladser af multiphoton mikroskopi. Angiogenese i genetisk modificerede stilladser blev studeret i en murine calvarial kritiske knogle defekt model. Flere nye blodkar blev opdaget i gruppen behandling end kontrol.

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Bioteknologi sag 127 knogledefekter knogledannelse 3D-PLGA/nHAp stillads modificerede genetisk angiogenese multiphoton mikroskopi
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