/

면역학 및 감염병학

/

支气管肺泡灌洗小鼠肺分析炎症细胞浸润

JoVE 신문
면역학 및 감염병학

This content is Free Access.

JoVE 신문 면역학 및 감염병학

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

Please note that all translations are automatically generated. Click here for the English version.

支气管肺泡灌洗小鼠肺分析炎症细胞浸润

DOI:

10.3791/55398-v

•

07:03 min

•

May 04, 2017

•

Lien Van Hoecke², Emma Richelle Job², Xavier Saelens², Kenny Roose²

¹UGent Center for Medical Biotechnology,VIB, ²Department of Biomedical Molecular Biology,Ghent University (UGent)

Chapters

00:05Title
00:52Broncoalveolar Lavage (BAL)
02:58BAL Fluid Component Collection and Immunostaining for Flow Cytometry (FACS)
04:27Results: Representative BAL Immune Cell Detection
06:17Conclusion

Summary

자동 번역

肺的健康状况的类型和存在于肺的细支气管的免疫细胞的数目反射。我们描述了支气管肺泡灌洗技术，它允许从小鼠的下呼吸道隔离和非粘附细胞和可溶性因子的研究。

Tags

Keywords: Bronchoalveolar Lavage Murine Lungs Inflammatory Cell Infiltration Respiratory Immunology Lung Infection Lung Inflammation Innate Cellular Response Humoral Response BAL Fluid Cell Harvesting Ex Vivo Analysis Disease Assessment

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

非手术治疗的小鼠气管内滴入肺和肺淋巴结流式细胞仪分析

肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集

全细胞MALDI-TOF质谱法是一种准确，快速的方法来分析巨噬细胞活化的不同模式

口咽部气管内PAMP管理和支气管肺泡灌洗的利用率来评估小鼠宿主的免疫反应

一种优化的协议分析和糖酵解线粒体呼吸的淋巴细胞

流量Virometry来分析病毒颗粒的抗原谱和外囊泡

从小鼠在原代肝细胞培养物的炎性反应的诱导

用于炎症反应的新内皮调节因子的筛选测定

慢性炎症状态个体的单核细胞轮回和泡沫形成的定量研究

一种新型大鼠自然杀伤细胞无饲养系统的研究体外

Read Article