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Biochemistry

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JoVE Journal Biochemistry
Verbesserte Sample Multiplexing von Gewebe mit Combined Precursor Isotopenmarkierung und Isobaric Tagging (cPILOT)
 
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Verbesserte Sample Multiplexing von Gewebe mit Combined Precursor Isotopenmarkierung und Isobaric Tagging (cPILOT)

Article DOI: 10.3791/55406-v • 09:06 min • May 1st, 2017
May 1st, 2017 •
1, 1, 2, 3, 1
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

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Kombinierte Vorläufer Isotopenmarkierung und isobar Tagging (cPILOT) ist eine quantitative Proteomik-Strategie, die Probe Multiplexierungsfähigkeiten von isobar-Tags verbessert. Dieses Protokoll beschreibt die Anwendung von cPILOT an Gewebe von einer Krankheit Mausmodell und Wildtyp-Kontrollen der Alzheimer.

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Biochemistry Heft 123 Quantitative Proteomik cPILOT TMT Isotopenmarkierung und isobar Tagging Multiplexing Gewebe
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