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Genetics

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JoVE Journal Genetics
Beoordeling van DNA besmetting in RNA Samples gebaseerd op Ribosomal DNA
 
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Beoordeling van DNA besmetting in RNA Samples gebaseerd op Ribosomal DNA

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Hier presenteren we een protocol voor het traceren van genomic DNA (gDNA) verontreiniging in RNA monsters. De onderhavige methode maakt gebruik van primers specifiek voor de interne getranscribeerde spacer regio (ITS) van ribosomal DNA (rDNA) genen. De methode is geschikt voor betrouwbare en gevoelige detectie van DNA besmetting in meeste prokaryoten en de eukaryoten.

Tags

Genetica kwestie 131 qPCR RT-qPCR gen expressie analyse DNA besmetting assay ribosomal DNA (rDNA) ribosomal RNA (rRNA) grassenfamilie prokaryote eukaryote universele primer
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