Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
 

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Article DOI: 10.3791/55610-v 10:23 min April 20th, 2017
April 20th, 2017

챕터

요약

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

Tags

Keywords: Microtubule Dynamics Yeast Cells GFP-labeled Tubulin Flow Chamber Slides Paraffin Film Time-lapse Imaging Microscopy Cell Biology Cytoskeleton
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter