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Neuroscience

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Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
 

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Article DOI: 10.3791/55627-v 10:36 min June 13th, 2017
June 13th, 2017

챕터

요약

Whole-cell recordings from Drosophila melanogaster photoreceptors enable the measurement of spontaneous dark bumps, quantum bumps, macroscopic responses to light, and current-voltage relationships under various conditions. In combination with D. melanogaster genetic manipulation tools, this method enables the study of the ubiquitous inositol-lipid signaling pathway and its target, the TRP channel.

Tags

Keywords: Electrophysiology Whole-cell Voltage Clamp Drosophila Photoreceptors Phototransduction TRP Channels Visual Transduction Dissection Calcium-free Extracellular Solution Tissue Preparation Inverted Microscope Perfusion System Suction System Ground Wire Electrophysiology Setup
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