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JoVE Journal Neuroscience
Assay zur Messung des nukleozytoplasmatischen Transports in Echtzeit in motorischen Neuronen-ähnlichen NSC-34-Zellen
 
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Assay zur Messung des nukleozytoplasmatischen Transports in Echtzeit in motorischen Neuronen-ähnlichen NSC-34-Zellen

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Dieses Protokoll beschreibt ein Verfahren zur sensitiven Messung der nukleozytoplasmatischen Transportrate in motorischen neuronähnlichen NSC-34-Zellen durch Quantifizierung der Echtzeitveränderung des nuklearen Importes eines NLS-NES-GFP-Proteins.

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Neurowissenschaften Ausgabe 123 nucleozytoplasmatischer Transport Shuttle-GFP NSC-34-Zellen C9orf72 ALS
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