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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
 

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Article DOI: 10.3791/55878-v 09:20 min August 10th, 2017
August 10th, 2017

챕터

요약

The present method serves to identify isoform-specific inhibitors of histone deacetylases (HDAC) in HeLa cells by the UHPLC-MS analysis of multiple substrates. This is an antibody-free method developed to reflect HDAC1 and HDAC6 activity in the living cell environment, in contrast to single-isoform cell-free assays.

Tags

Keywords: HDAC1 HDAC6 HeLa Cells UHPLC-MS HDAC Inhibitor Selectivity Drug Discovery Simultaneous Measurement Cell-based Assay Acetylation Trichostatin A MS275 Tubastatin A
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