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自噬研究中蛋白质-蛋白质相互作用的研究
JoVE 신문
생물학
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JoVE 신문 생물학
Study of Protein-protein Interactions in Autophagy Research
DOI:

14:08 min

September 09, 2017

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Chapters

  • 00:00Title
  • 01:25Immunofluorescence
  • 05:43Representative Results for Immunofluorescence
  • 06:16Immunoprecipitation
  • 06:38Harvesting Cells
  • 07:33Protein Isolation
  • 08:10Bradford Assay
  • 08:36Antibody Coupling of Beads
  • 09:26Incubation of Total Cell Lysate with Beads
  • 09:52Elution of Proteins from Beads
  • 10:18Preparation of Input Controls
  • 10:43Denaturation of Proteins and Western Blot
  • 13:06Representative Results for Immunoprecipitation
  • 13:29Conclusion

Summary

자동 번역

本文介绍了两种抗体蛋白-蛋白质相互作用的研究方法: 免疫荧光和沉淀。这些技术适于研究蛋白质之间的物理相互作用, 以发现细胞信号通路的新成分, 以及了解蛋白质动力学。

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