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Hemoglobinopathies 患者造血干细胞移植后残余供红祖细胞的检测

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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

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Hemoglobinopathies 患者造血干细胞移植后残余供红祖细胞的检测

DOI:

10.3791/56002-v

•

11:59 min

•

September 06, 2017

•

Roman Crazzolara, Gabriele Kropshofer, Michael Steurer, Sieghart Sopper³, Wolfgang Schwinger

¹Department of Pediatrics,Medical University Innsbruck, ²Department of Internal Medicine V (Hematology & Oncology),Medical University Innsbruck, ³Tyrolean Cancer Research Institute, ⁴Department of Pediatrics,Medical University Graz

Chapters

00:05Title
01:22Isolation of Hematopoietic Bone Marrow cells by Multi-parameter Fluorescence-Actviated Cell Sorting (FACS)
06:33Clonogenic Assay
07:54Analysis of Chimerism by PCR
09:16Results: Quantifying Relative Proportions of Donor-versus-recipient Chimerism in the Erythroid Progenitors
10:54Conclusion

Summary

자동 번역

对 hemoglobinopathies 患者进行干细胞移植后, 需要对供体细胞进行定量监测植入。流 cytometry-based 细胞分选、菌落形成检测和随后的短串联重复分析的结合可用于评估红室中祖体的增殖和分化。

Tags

Keywords: Hematopoietic Stem Cell Transplantation Hemoglobinopathies Chimerism Erythroid Progenitor Cells Flow Cytometry Short Tandem Repeats Graft Rejection Disease Relapse Immunomodulatory Strategies Cell Suspension Bone Marrow Gradient Medium Mononuclear Cells Cell Concentration Trypan Blue Stain Hemocytometer

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