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JoVE Journal Developmental Biology
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
 

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

Article DOI: 10.3791/56039-v • 10:57 min • September 12th, 2017
September 12th, 2017 •
1, 1, 1, 2,3, 1
1Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, 2Department of Neurology, Yale University School of Medicine, 3Department of Neurology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine

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요약
Automatic Translation

Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.

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Keywords: Aorta Vascular Development Smooth Muscle Supravalvular Aortic Stenosis Embryonic Perinatal Morphogenesis Pathogenesis In Vivo Tissue Explant Cell Explant Cre ER Tamoxifen Progesterone Paraformaldehyde Sucrose OCT Compound Cryosectioning
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