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C. 线虫排泄管作为细胞内腔形态发生和体内极化膜生物在单个细胞中的模型: GFP-融合、rna 干扰界面和成像的标记
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The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
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C. 线虫排泄管作为细胞内腔形态发生和体内极化膜生物在单个细胞中的模型: GFP-融合、rna 干扰界面和成像的标记

DOI:
10.3791/56101-v

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10:30 min

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October 03, 2017

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, , , , ,
1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children,Harvard Medical School, 2College of Life Sciences,Jilin University, 3Faculty of Health Sciences,University of Macau

Chapters

  • 00:05Title
  • 01:02Introduction to the Accompanying Publication
  • 02:28RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension
  • 03:29Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy
  • 05:20Imaging the C. elegans Excretory Canal using Confocal Microscopy
  • 08:16Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes
  • 09:51Conclusion

Summary

자동 번역

자동 번역

C. 线虫排泄管是一种独特的单细胞模型, 用于可视化的体内分析从头生物极化膜。该协议描述了标准的遗传/rna 干扰和成像方法相结合, 适用于定向单细胞 tubulogenesis、顶端膜和腔生物的分子的鉴定和表征。

Tags

Keywords: C. ElegansExcretory CanalIntracellular LumenPolarized Membrane BiogenesisTubulogenesisPolarityGFP FusionRNAiLive ImagingMembrane MarkersOrganelle MarkersPromotersERM-1Cystic Canal PhenotypeModifier Screen

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