Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Laboratory Protocol for Genetic Gut Content…
JoVE Journal
Biology

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biology
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
 

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Article DOI: 10.3791/56132-v • 10:17 min • October 5th, 2017
October 5th, 2017 •
1,2, 1,3
1Institute for Environmental Sciences, University of Koblenz-Landau, 2Institute of Integrated Natural Sciences, University of Koblenz-Landau, 3IV 2.5 (Trace Analysis, Artificial Pond and stream system), Federal Environment Agency

챕터

요약
Automatic Translation

Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.

Tags

Keywords: Genetic Gut Content Analysis Aquatic Macroinvertebrates RDNA Primers Trophic Ecology Food Web Invasive Species DNA Extraction Proteinase K SDS Sodium Chloride Isopropanol PCR Primer Design
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter