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1School of Biomedical Sciences & Curtin Health Innovation Research Institute,Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute,Curtin University, 3Institute of Biochemistry,Christian-Albrechts-University, 4School of Veterinary and Life Sciences,Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology,The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences,The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences,Edith Cowan University, 12School of Medicine and Pharmacology,University of Western Australia
Chapters
- 00:00Title
- 01:21Choline-deficient and ethionine-supplemented diet treatment
- 02:27Liver perfusion and isolation
- 04:53Results: Representative CDE chronic liver injury model dynamics
- 06:51Conclusion
Hier beschreiben wir eine gängige Methode um chronische Schädigung der Leber bei Mäusen zu induzieren, durch die Fütterung einer Cholin-Mangel und Ethionine ergänzt (CDE) Ernährung. Wir zeigen Systemüberwachung, Leber Perfusion, Isolation und Erhaltung. Schädigung der Leber, Pathohistology, Fibrose, entzündliche, und Leber Stammvater-Zell-Reaktionen kann ein zeitlichen Verlauf von sechs Wochen mitteilen.
