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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
 

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

Article DOI: 10.3791/56144-v 11:33 min August 17th, 2017
August 17th, 2017

챕터

요약

A new generation of cell-free transcription-translation platforms has been engineered to construct biochemical systems in vitro through the execution of gene circuits. In this article, we describe how bacteriophages, such as MS2, ΦΧ174, and T7, are synthesized from their genome using an all E. coli cell-free TXTL system.

Tags

Keywords: Bacteriophage Synthesis E. Coli Cell-free Expression System Phage Plaques Phage Infection DNase Sucrose Gradient Ultracentrifugation Phage Band Extraction
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