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游离的小鼠皮层神经元文化的制备

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Preparation of Dissociated Mouse Cortical Neuron Cultures

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游离的小鼠皮层神经元文化的制备

DOI:

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18:02 min

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December 19, 2007

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Lutz G. W. Hilgenberg, Martin A. Smith

¹Department of Anatomy and Neurobiology,University of California, Irvine (UCI)

Chapters

00:00Title
00:15Introduction
01:06Tools and Reagents
03:13Mounting Brain onto Vibraslicer
04:26Removing and Sectioning Brain
08:12Isolating and Processing Cortex
11:29Washing Cortical Pieces
13:14Trituration of Cortical Pieces
16:35Visualizing Cortical Neurons
17:12Conclusion

Summary

자동 번역

这个视频显示了从胚胎后期和产后早期小鼠皮层神经元的文化生成的过程。这些文化可用于免疫细胞化学，生物化学，电生理，钙和钠成像，并提供一个平台，携带产后的致死基因突变的转基因动物研究神经元发育。

Tags

Dissociated Mouse Cortical Neuron Cultures Late Embryo Early Postnatal Mouse Brain Immunocytochemistry 생화학 Electrophysiology Calcium Imaging Sodium Imaging Protein Isolation RNA Isolation Transgenic Animals Gene Mutation Cortical Rind Thalamo-cortical Fiber Tract Non-neuronal Cells Neuronal Cells Enzyme Incubation Step Glia Feeder Cells

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