Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Dissection of Human Retina and RPE-Choroid for…
JoVE Journal
Biochemistry
Author Produced

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biochemistry
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
 

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Article DOI: 10.3791/56203-v • 06:55 min • November 12th, 2017
November 12th, 2017 •
*1,2,7,8, *3,4, 3,4, 1,2, 3, 3, 1,2, 5,6, 3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
* These authors contributed equally

챕터

요약
Automatic Translation

The human retina is composed of functionally and molecularly distinct regions, including the fovea, macula, and peripheral retina. Here, we describe a method using punch biopsies and manual removal of tissue layers from a human eye to dissect and collect these distinct retinal regions for downstream proteomic analysis.

Tags

Keywords: Retina RPE-choroid Proteomic Analysis Fovea Macula Peripheral Retina Punch Biopsy Colibri Forceps Westcott Scissors Tissue Collection Vitreous Gel Sclera One Dimensional STS Page
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter