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1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons,Columbia University, 2Edward S. Harkness Eye Institute,New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology,Stanford University, 4Medical Scientist Training Program,University of Iowa, 5Department of Pediatrics,University of Iowa, 6Department of Neurology,University of Iowa, 7Department of Ophthalmology,Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology,Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
Chapters
- 00:00Title
- 01:15Retina Dissection
- 03:00RPE-Choroid Dissection
- 04:39Scissors Dissection
- 05:15Representative Results
- 06:11Conclusions
Die menschliche Netzhaut besteht aus funktional und molekular verschiedene Regionen, einschließlich der Fovea, der Makula und der peripheren Netzhaut. Hier beschreiben wir eine Methode mit Stanzbiopsien und manuelle Entfernung von Gewebeschichten von einem menschlichen Auge zu zerlegen und diese verschiedenen retinalen Regionen für nachgeschaltete Proteomic Analysen zu sammeln.
