Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Sample Preparation for Endopeptidomic Analysis in …
JoVE Journal
Neuroscience

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Neuroscience
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
 

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Article DOI: 10.3791/56244-v • 10:23 min • December 4th, 2017
December 4th, 2017 •
1, 1,2, 1, 1, 1, 1,2, 1,2,3, 1,2
1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3Department of Molecular Neuroscience, UCL Institute of Neurology

챕터

요약
Automatic Translation

A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.

Tags

Keywords: Endopeptidomic Analysis Cerebrospinal Fluid Sample Preparation LC-MS Peptide Pre-fractionation Endogenous Peptides Biomarkers Neurodegenerative Disorders MWCO Filtration High-pH Reverse Phase HPLC Mass Spectrometry
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter